June 2008

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Journal of Applied Animal Research

Vol. 33 No. 2      June, 2008

(Abstracted/indexed in AGRIS Database, Biosis Database, CAB Abstracts,Chemical Abstracts, CURRENT CONTENTS (AB and ES), Food Science and Technology Abstracts, Indian Science Abstracts, RESEARCH ALERT, SCISEARCH)

TOPIC AUTHOR

Effect of sunn hemp seed inclusion in broiler starter diets on live performance attributes

J.B. Hess and J.A. Mosjidis (USA)

Dietary quality and safety in the reuse of broiler litter as a feed ingredient through fermentation and pelleting

T. Suppadit , K. Parukatpichai and N. Talakhun (Thailand)

The acceptability of goat milk to young Japanese adults

Takeyuki Ozawa, Jiro Nishitani (Japan), Nicolas Lopez-Villalobos and Hugh Blair (New Zealand)

Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos

Santosh Haunshi, S.C. Saxena, A. Pattanayak, S. Bandyopadhaya, A.K. Tyagi and K. M. Bujarbaruah (India)

Effect of antagonistic minerals (Fe, S and Zn) on absorption and metabolism of Cu by sheep fed poultry waste

A.F. Castellanos-Ruelas, J.A. Pacheco-Aguirre, M.L. Murguía-Olmedo, J.G. Rosado-Rubio, D.A. Betancur-Ancona and L.A. Chel-Guerrero (Mexico)

Litter beetle (Alphitobius diaperinus Panzer) counts in broiler houses treated with a range of insecticides

J.B. Hess, K.S. Macklin and B.A. McCrea (USA)

Responses to sweetener quinine mixtures in chicks: short-term fluid intake test

H. Furuta, T. Izumi, K. Dodo, K. Yahata, S. Nishimoto and T. Bungo (Japan)

Estimation of endogenous nitrogen and amino acids flow at the duodenum and ileum in growing goats fed on different NDF level diets

C.S. Zhou, H.L. Jiang, Z.L. Tan, X.G. Zhao, Z.H. Sun, S.X. Tang and M. Wang (China)

Determination of genetic distance between East Anatolian Red, Brown Swiss, Holstein and Simmental breeds of cattle using polymorphic systems

U. Dogru, M. Ozdemir, M.I. Soysal and M. Topal (Turkey)

A new method of brain staining with triphenyltetrazolium chloride to determine the infarct size in rats

E. Kheradpezhouh, R. Miri, A. Noorafshan, M.R. Panjehshahin and D. Mehrabani (Iran)

Dry matter and nitrogen degradation parameters of tropical tree foliages eaten by West African dwarf sheep

I. Ikhimioya, A.O. Isah, K.O. Akhidenor and E. Otite (Nigeria)

Genetic diversity in river buffalo (Bubalus bubalis) breeds of central India using heterologous bovine microsatellite markers

B.P. Mishra, R.S. Kataria, S.S. Bulandi, V. Kumar and M. Mukesh (India)

A nonlinear regression model for growth

S. Prasad, R.K. Singh and R. Singh (India)

Effect of dietary crude protein level on quality and production of meat in Holstein steers

A. Pasinato, J. Elizalde, O. Tisocco, G. Teira, F. Perlo and P. Bonato (Argentina)

Egg production and quality in laying hens fed on different digestible amino acid regimes and housed at three stocking densities

B. Vázquez-González, L. Sarmiento-Franco, R. Santos-Ricalde, M. Carvajal-Hernández, J. Segura-Correa, M. Gutiérrez-Triay (Mexico)

Foetal monitoring, uterine dynamics and reproductive performance in spontaneous farrowings in sows

A. Olmos-Hernández, M.E. Trujillo-Ortega, M. Alonso-Spilsbury, P. Sánchez-Aparicio, R. Ramírez-Necoechea and D. Mota-Rojas (Mexico)

Effects of feeding phytogenic substances and phytase on growth perfomrnace and nutrient digestibility of young broilers

T.C. Loh, H.G. Ling, N.T. Thanh, H.L. Foo, M.A. Rajion and S.I. David (Malaysia)

Mechanistic model evaluation of tree species and cactae commonly consumed by grazing goats

L. Vargas-Villamil, A.S. Juárez-Reyes, J. Ku-Vera, E. Ávila-Vales, F. Vargas-Villamil, S. Medina-Peralta and M.A. Cerrillo-Soto (Mexico)

Gestation duration and rectal temperature changes during peripartum period in dogs A.K. Zonturlu, O.A. Aksoy and C. Kacar (Turkey)

Laparoscopy for direct examination of internal organs in small animals

S.K. Maiti, A. Dutta, P. Ajith, N. Kumar, A.K. Sharma and G.R. Singh (India)

Molecular variability of somatotropin releasing hormone (SRH) gene in Mithun (Bos frontalis)

S. Kumar, R.P. Manohar V, S.M. Deb, A. Mitra, B. John, A. Sharma and K.M. Bujarbaruah (India)

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Effect of Sunn Hemp Seed Inclusion in Broiler Starter Diets on Live Performance Attributes

J.B. Hess1, J.A. Mosjidis2

1Poultry Science Department

2Department of Agronomy and Soils

Auburn University

AL 36849-5416, USA

(Received June 21, 2007; accepted January 23, 2008)

Abstract

Hess, J.B. and Mosjidis, J.A. 2008. Effect of sunn hemp seed inclusion in broiler starter diets on live

performance attributes. J. Appl. Anim. Res., 33: 105-108.

 

This trial examined live performance responses of mixed-sex broilers raised to 21 d of age in two trials to determine whether sunn hemp seeds of a breeding population containing 2.5% of the pyrrolizidine alkaloids present in showy crotalaria were detrimental to young broilers. In each trial, the birds were housed in Petersime battery brooders with eight replicates per treatment (a total of 240 birds per trial). In trial 1, mash starter feed was fed to 21 d with or without whole sunn hemp seed. Treatments included a control, a contamination level (0.5% sunn hemp seed) and an ingredient inclusion level (5% sunn hemp seed). In the second trial, ground sunn hemp was included in starter feeds at 0.5% and 2% in addition to a control. Body weights and feed consumption were reduced in birds fed 5% or 2% sunn hemp (with poorer FCR), but not in birds fed 0.5% as compared to the control. No significant mortality differences were measured between treatments and no unusual pathologies were noted in the birds during posting. Low level feed contamination with sunn hemp did not measurably reduce broiler live performance during the starter period.

 

Key words: Sunn hemp, crotalaria, broiler, poultry.

Dietary Quality and Safety in the Reuse of Broiler Litter as a Feed Ingredient through Fermentation and Pelleting

T. Suppadit* , K. Parukatpichai1, N. Talakhun2

The Graduate Program in Environmental Management

School of Social and Environmental Development

National Institute of Development Administration

Bangkapi, Bangkok 10240, Thailand

(Received June 8, 2007; accepted January 25, 2008)

Abstract

Suppadit, T., Parukatpichai, K. and Talakhun, N. 2008. Dietary quality and safety in the reuse of broiler litter as a feed ingredient through fermentation and pelleting. J. Appl. Anim. Res., 33: 109-112.

To modify broiler litter (BL) by fermentation and/or pelleting as a feed ingredient, BL samples were collected from twenty randomly selected farms in Saraburi province, Thailand in December, 2006. All the farms used rice husks as the bedding materials. Prior to and after the modifications, the nutritive values and health risks were assessed. After fermentation, most of the nutrient contents tended to decrease (P<0.05). By contrast, moisture and gross energy content increased (P<0.05). Pelleting did not alter the nutrient content of the BL. Parasites, aflatoxins and antibiotics were not found in any BL samples. Pathogenic microorganisms, heavy metals and sodium chloride were detected in all samples. All pathogens and heavy metals decreased after processing. In conclusion, fermentation followed by pelleting could eliminate health risks and increase the potential scope for utilizing BL as an animal feed.

Key words: Broiler litter, diet, fermentation, nutritive value, pelleting, safety.

The Acceptability of Goat Milk to Young Japanese Adults

Takeyuki Ozawa1, Jiro Nishitani1, Nicolas Lopez-Villalobos2, Hugh Blair2

1Faculty of Animal Science

Nippon Veterinary and Life Science University,

1-7-1 Kyonan-cho, Musashino-shi

Tokyo, 180-8602 Japan

2Institute of Veterinary

Animal and Biomedical Sciences

Massey University, Palmerston North

New Zealand

(Received August 14, 2007; accepted February 6, 2008)

Abstract

Ozawa, T., Nishitani, J., Lopez-Villalobos, N. and Blair, H. 2008. The acceptability of goat milk to young Japanese adults. J. Appl. Anim. Res., 33: 113-116.

For sensory evaluation of fresh goat milk, a sample of 58 students from the Nippon Veterinary and Life Science University were randomly and evenly divided into two groups as “Group I” and “Group II”. Only Group II received a 10-min lecture about the health benefits of goat milk. Bottles of commercial goat milk were used for the tasting. Each participant was offered 100 ml of fresh goat milk and was given the option to leave residual milk if the flavour was not considered to be satisfactory. Milk was graded for taste, smell, richness and overall evaluation. Wilcoxon rank-sum test, Chi-squared analyses and one-way analysis of variance were carried out to search for significant differences between the two groups. Overall, goat milk was found to be moderately acceptable to both groups of trial participants. However, the amount of residual milk was lower in Group II compared to Group I (P<0.05). Thus goat milk was moderately acceptable to young Japanese adults. In addition, promoting the health benefits of goat milk to consumers via written and verbal information can significantly increase the amount of goat milk consumption.

Keywords: Goat milk, sensory test, promotion, Japan.

Simple Multiplex PCR for Rapid Diagnosis of Sex of Ducks and Duck Embryos

Santosh Haunshi,* S.C. Saxena, A. Pattanayak1, S. Bandyopadhaya2,

A.K. Tyagi3, K. M. Bujarbaruah4

 

Molecular Genetics Laboratory

Division of Poultry Science

ICAR Research Complex for N.E.H. Region

Umroi Road, Umiam-793 103, India

(Received June 21, 2007; accepted February 4, 2008)

Abstract

Haunshi, S., Saxena, S.C., Pattanayak, A., Bandyopadhaya, S., Tyagi, A.K. and Bujarbaruah, K.M. 2008. Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos. J. Appl. Anim. Res., 33: 117- 120.

A simple and rapid multiplex polymerase chain reaction (PCR) was developed for quick diagnosis of sex of duck and duck embryos. W chromosome specific DNA sequence was selected and primers were designed to amplify 335 bp fragment from female sex while 16S ribosomal sequence was selected to design primers to amplify 468 bp PCR products both in male and female sex as an internal control. Nucleotide sequences of W chromosome specific DNA fragments of Khaki Campbell and Indian Runner breeds of duck were found to be identical both in size and sequences. The study concluded that the protocol was successful in precisely identifying the gender of ducklings belonging to both Indian Runner and Khaki Campbell breeds of ducks and duck embryos.

Key words: Multiplex PCR, duck, embryo, sex diagnosis.

Effect of Antagonistic Minerals (Fe, S and Zn) on Absorption and Metabolism of Cu by Sheep Fed Poultry Waste

A.F. Castellanos-Ruelas*, J.A. Pacheco-Aguirre, M.L. Murguía-Olmedo1, J.G. Rosado-Rubio,

D.A. Betancur-Ancona, L.A. Chel-Guerrero

 

Facultad de Ingeniería Química

Universidad Autónoma de Yucatán

Av. Juárez # 421. Ciudad Industrial. C.P. 97288. Mérida, Yuc. México

 

(Received November 29, 2006; accepted February 23, 2008)

Abstract

Castellanos-Ruelas, A.F., Pacheco-Aguirre, J.A., Murguía-Olmedo, M.L., Rosado-Rubio, J.G., Betancur-Ancona, D.A. and Chel-Guerrero, L.A. 2008. Effect of antagonistic minerals (Fe, S and Zn) on absorption and metabolism of Cu by sheep fed poultry waste. J. Appl. Anim. Res., 33: 121-125.

To examine effects of feeding higher levels of minerals antagonistic to Cu (S, Fe and Zn) on production and digestibility of Cu of hair sheep fed a diet based on poultry waste (poultry manure) containing over 90 ppm Cu for 90 d, eight diets were fed to thirty-two male Pelibuey sheep. The diets contained each of the three minerals (Fe, S and Zn) at two levels, 647 mg/kg, 0.39% and 216 mg/kg; versus 833 mg/kg, 0.42% and 772 mg/kg, respectively in a 2x2x2 factorial design. Animal productivity as well as Cu content of liver and kidney were not affected by the treatments. High levels of Fe reduced (P<0.05) Cu digestibility. The ceruloplasmin activity during the experiment also was reduced by the addition of S or Fe plus Zn. Results indicate that supplementation with S or Fe plus Zn may delay Cu intoxication of sheep consuming diets containing excess Cu.

Keywords: Poultry manure, copper, absorption, minerals, hair sheep.

Litter Beetle (Alphitobius diaperinus Panzer) Counts in Broiler Houses Treated with a Range of Insecticides

J.B. Hess1, K.S. Macklin1, B.A. McCrea2

1Poultry Science Department

Auburn University, AL 36849-5416

2University of California at Davis

Davis, CA

USA

(Received August 8, 2007; accepted January 21, 2008)

Abstract

Hess, J.B., Macklin, K.S. and McCrea, B.A. 2008. Litter beetle (Alphitobius diaperinus Panzer) counts in broiler houses treated with a range of insecticides. J. Appl. Anim. Res., 33:127-131.

Litter beetles (Alphitobious diaperinus Panzer) remain an important poultry production pest on several fronts. They have been shown to damage poultry houses and harbor pathogens that affect people and poultry. This trial examined beetle counts in commercial broiler houses treated with 1 of 2 insecticide treatments, applied dry or wet. Three levels of dry (12.20, 15.26 and 18.31 g/m2) and one level of liquid application of an organophosphate (Rabon) and pyrethroid (Tempo) dry (1% dust) and wet (20% Wettable powder) were compared to an untreated control house across one growout. Six pvc pipes (2.5 cm wide by 25 cm long) were filled with a rolled up piece of chick paper. They were placed in each house for a 3 d period during brooding, at 3 wk of age and prior to marketing the birds. The tubes were placed in plastic bags and refrigerated for 24 h prior to counting larval and adult litter beetles. All treatments reduced larval and adult litter beetles in relation to the untreated control house. The organophosphate did not reduce adult beetle numbers as quickly as pyrethroid, but showed good control by the 3rd week. As expected, the 15.26 and 18.31 g/m2 application rates of the organophosphate reduced beetle numbers, while the 12.20 g level did not control beetles as well. Products applied dry showed better control than those applied as a liquid, with larvae levels increasing as the flock aged in the liquid treatment. The products tested controlled litter beetles at suggested application rates, with dry applications showing better control than liquid applications.

Key words: Litter beetle,insecticide, broiler.

Responses to Sweetener-Quinine Mixtures in Chicks: Short-term Fluid Intake Test

H. Furuta*1, T. Izumi2, K. Dodo2, K. Yahata2, S. Nishimoto2, T. Bungo3

1Department of Animal Science

Nippon Veterinary and Life Science University

Musashino Tokyo 180-8602, Japan

2Department of Agrobiological Science

Faculty of Agriculture

Ehime University

Matsuyama Ehime 790-8566, Japan

3Department of Bioresource Science

Graduate School of Biosphere Science

Hiroshima University

Higashi-Hiroshima Hiroshima 739-8528, Japan

(Received September 4, 2007; accepted April 5, 2008)

Abstract

Furuta, H., Izumi, T., Dodo, K., Yahata, K., Nishimoto, S. and Bungo, T. 2008. Responses to sweetener quinine mixtures in chicks: short-term fluid intake test. J. Appl. Anim. Res., 33: 133-136.

To study behavioral responses to sweet taste stimuli in young chickens, White leghorn chicks were given mixed-solutions of quinine and saccharin or glycine for 10 minutes after being deprived of water for 6 h. The consumption of quinine alone solution was significantly lower than water control (p<0.05), whereas saccharin (1.0 mM) or glycine (0.5 M) produced a recovery from taste aversion of quinine to approximately 82-83% of baseline (control) level. Our results suggest that chicks seem to sense sweetness and this shortterm test may be a useful tool to evaluate taste sense for chicks.

Key words: Taste, saccharin, quinine, glycine, water intake, chick.

Estimation of Endogenous Nitrogen and Amino Acids Flow at the Duodenum and Ileum in Growing Goats Fed on Different NDF Level Diets

C.S. Zhou1,2, H.L. Jiang1,2, Z.L. Tan1*, X.G. Zhao1,2, Z.H. Sun1,2, S.X. Tang 1,2, M. Wang1

1Key Laboratory of Subtropical Agro-ecological Engineering

Institute of Subtropical Agriculture

The Chinese Academy of Sciences

Changsha Hunan 410125-10, P. R. China

2Graduate University of the Chinese Academy of Sciences

Beijing 100039, P. R. China

(Received July 24, 2007; accepted April 4, 2008)

Abstract

Zhou, C.S., Jiang, H.L., Tan, Z.L., Zhao, X.G., Sun, Z.H., Tang, S.X. and Wang, M. 2008. Estimation of endogenous nitrogen and amino acids flow at the duodenum and ileum in growing goats fed on different NDF level diets. J. Appl. Anim. Res., 33: 137-144.

An experiment was conducted with four ruminally, duodenally and ileally cannulated goats (15±0.5 kg) allocated to four dietary treatments in a 4×4 Latin square design to estimate the effects of dietary NDF levels on the losses of endogenous nitrogen (EN) and endogenous amino acids at the duodenum and ileum in goats. Goats were fed the total mixed diets containing four levels of NDF (39.69, 36.95, 34.22 and 31.49%). The duodenal flow of EN and AA was determined by the difference and amino acid profile (AAP) method, respectively. The ileal flow of EN and AA was measured by the water soluble protein method. The duodenal flow of EN estimated by the two methods were not affected (P>0.05) by the dietary NDF content. For the estimated values by the AAP method, the duodenal flow of endogenous AA expressed less variation than those by the difference method. When compared with the difference method, the duodenal flow and the re-absorption of endogenous N and AA were higher than those estimated by the AAP method. The results implied that dietary fiber content might influence the secretion of endogenous protein and the more reasonable estimates of endogenous N and AA could be obtained by the AAP method.

Keywords: Endogenous protein, NDF, amino acid profile, goat.

Determination of Genetic Distance Between East Anatolian Red, Brown Swiss, Holstein and Simmental Breeds of Cattle Using Polymorphic Systems

Unsal Dogru1*, Memis Ozdemir1, Mehmet Ihsan Soysal2, Mehmet Topal1

1Department of Animal Science

College of Agriculture, Ataturk University

25240 Erzurum, Turkey

2Department of Animal Science

Faculty of Agriculture, Namik Kemal University

59030 Tekirdag, Turkey

(Received March 24, 2007; accepted March 14, 2008)

Abstract

Dogru, U., Ozdemir, M., Soysal, M.I. and Topal, M. 2008. Determination of genetic distance between East Anatolian Red, Brown Swiss, Holstein and Simmental breeds of cattle using polymorphic systems. J. Appl. Anim. Res., 33: 145-148.

Genetic distances were determined according to Nei, Cavalli-Sforza and Reynolds methods between the Brown Swiss (173), Simmental (80), Holstein (18) and East Anatolian Red (51) cattle breeds using milk protein (αs1) and blood protein types (Hb and Tf). The distribution of phenotypic frequencies in all the four breeds for all systems except αs1-Cn was significant (P<0.01) using chi-square test. Phylogenetic trees based on the polymorphic systems of the populations obtained by three different methods of Nei, Cavalli-Sforza and Reynolds were found to be similar. According to the phylogenetic trees Brown Swiss and Simmental cattle breeds fall in one group and dairy (Holstein) and Native (East Anatolian Red) breeds in different groups.

Keywords: Milk protein loci, transferrin, haemoglobin loci, phylogenetic tree.

A New Method of Brain Staining with Triphenyltetrazolium Chloride to Determine the Infarct Size in Rats

E. Kheradpezhouh1,2*, R. Miri2, A. Noorafshan3, M.R. Panjehshahin1, D. Mehrabani4

1Department of Pharmacoloy

2Medicinal and Natural Products Chemistry Research Center

3Department of Anatomy

4Gastroenterohepatology Research Center

Nemazee Hospital, Shiraz Medical School

Shiraz University of Medical Sciences

Shiraz, 71955-848 Iran

(Received March 22, 2007; accepted March 18, 2008)

Abstract

Kheradpezhouh, E., Miri, R., Noorafshan, A., Panjehshahin, M.R. and Mehrabani, D. 2008. A new method of brain staining with triphenyltetrazolium chloride to determine the infarct size in rats. J. Appl. Anim. Res., 33: 149-152.

To show the accuracy of 2, 3, 5-Triphenyltetrazolium chloride (TTC) staining method, in a case-control study, 10 rats were divided into two groups, an ischemia-induced (experimental) and a control group. The brains of both groups were stained with the new method. The complete staining of the brain of all rats in the control group confirmed the efficacy of this method. Besides, in the experimental group, the method distinguished between the viable and dead tissues and could demonstrate the borders and the size of the infarction too.

Key words: 2, 3, 5-triphenyltetrazolium chloride (TTC), cerebral ischemia, stereology.

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Dry Matter and Nitrogen Degradation Parameters of Tropical Tree Foliages Eaten by West African Dwarf Sheep

I. Ikhimioya#, A.O. Isah, K.O. Akhidenor, E. Otite

Department of Animal Science

Faculty of Agriculture

Ambrose Alli University

Ekpoma, Nigeria

(Revised received January 27, 2008; accepted February 12, 2008)

Abstract

Ikhimioya, I., Isah, A.O., Akhidenor, K.O. and Otite, E. 2008. Dry matter and nitrogen degradation parameters of tropical tree foliages eaten by west African dwarf sheep. J. Appl. Anim. Res., 33: 153-158.

Foliages from Ficus exasperata, Spondias monbin, Tectonia grandis and Terminalia catappa were evaluated to measure rumen degradation characteristics of their DM and N contents using nylon bag technique. Effective and potential degradabilities of dry matter of F. exasperata were much higher than those of other trees. Whereas, effective and potential degradabilities of N were highest for F. exasperata and lowest for T. catappa. The leaves of S. monbin, T. grandis and T. catappa appeared to be potential sources of rumen undegraded protein to the small intestine which could probably be exploited for the benefits of ruminants.

Keywords: Tropical tree foliages, degradation, dry matter, nitrogen, ruminants, chemical composition.

Genetic Diversity in River Buffalo (Bubalus bubalis) Breeds of Central India using Heterologous Bovine Microsatellite Markers

B.P. Mishra*, R.S. Kataria, S. S. Bulandi, V. Kumar, M. Mukesh

Buffalo Genomics Laboratory

National Bureau of Animal Genetic Resources

Karnal-132 001, India

(Received May 12, 2007; accepted March 06, 2008)

Abstract

Mishra, B.P., Kataria, R.S., Bulandi, S.S., Kumar, V. and Mukesh, M. 2008. Genetic diversity in river buffalo (Bubalus bubalis) breeds of central India using heterologous bovine microsatellite markers. J. Appl. Anim. Res., 33: 159-163.

A panel of 25 bovine specific heterologous microsatellite markers was employed for evaluating both within and between breed genetic variability estimates in Pandharpuri and Nagpuri buffaloes, two prominent breeds of Central India. A total of 148 alleles were detected across the two breeds with mean number of 5.92 alleles. The mean allelic diversity and heterozygosity reflected the existence of relatively higher genetic variability in Nagpuri buffalo. The F-statistics estimates were significantly different from zero (P<0.05) with F (FIT) = 0.349 ± 0.051, f (FIS) = 0.254 ± 0.039, θ (FST) = 0.126 ± 0.042. Most of the genetic variations correspond to differences among individuals (87.4%). Various genetic differentiation estimates indicated moderate differentiation between Nagpuri and Pandharpuri buffaloes. Individual assignment and interindividual allele sharing estimates also substantiated the presence of discrete genetic structure in both the buffalo breeds from Central India.

Key words: Indian river buffalo, genetic diversity, microsatellite markers.

A Nonlinear Regression Model for Growth

Shiv Prasad, Ram Karan Singh, Rajendra Singh

Division of Livestock Economics

Statistics and Information Technology

Indian Veterinary Research Institute

Izatnagar-243 122, India

(Received December 2, 2006; accepted December 16, 2007)

Abstract

Prasad, S., Singh, R.K. and Singh, R. 2008. A nonlinear regression model for growth. J. Appl. Anim. Res., 33: 165-167.

The polynomial regressions are not suitable to describe the growth in which the dependent variable approaches to an asymptotic value as independent variable passes through infinity. The Stevens model is, generally, used to model the asymptotic growth. A nonlinear regression model that is an extension of this model is proposed in this study and empirically has been shown that the proposed model performs better than Stevens model for estimation of growth curves.

Key words: Asymptotic growth, nonlinear regression model, Stevens model.

Effect of Dietary Crude Protein Level on Quality and Production of Meat in Holstein Steers

A. Pasinatoa*, J. Elizaldeb, O. Tisoccoc, G. Teirac, F. Perloc, P. Bonatoc

aNational Institute of Agricultural Technology (INTA)

Research Station Concepción del Uruguay

CC6 (3260) Entre Ríos, República Argentina`

bNational University of Mar del Plata

Faculty of Agriculture, C.C.276

(7620) Balcarce, Buenos Aires, República Argentina

cMeat Industries Laboratory

Sciences Feeding Faculty, Entre Ríos National University (UNER)

M Tavella 1450, (3200) Concordia, Entre Ríos, República Argentina.

(Received August 10, 2007; accepted April 06, 2008)

Abstract

Pasinato, A., Elizalde, J., Tisocco, O., Teira, G., Perlo, F. and Bonato, P. 2008. Effect of dietary crude protein level on quality and production of meat in Holstein steers. J. Appl. Anim. Res., 33: 169-173.

To characterize the effect of dietary crude protein level on quality and production of meat in Holstein steers, they received complete diets with the same metabolizable energy concentration but different protein content: 12, 14 and 17%, respectively. The average daily live weight gain was 1.32±0.17 kg/head.day-1 without differences among treatments (P>0.05). The group that received the highest level of protein consumed more dry matter (P<0.05) and had lower feed conversion than the group of animals with the diet with 12% crude protein. There were no differences (P>0.05) in the chemical composition of meat among the three treatments. The pH values of all the animals were in the range of an optimum value (5.54 to 5.70). There were no marked effects of the dietary protein level on meat production and quality.

Keywords: Meat quality, dietary protein level, Holstein steers.

Egg Production and Quality in Laying Hens Fed on Different Digestible Amino Acid Regimes and Housed at Three Stocking Densities

B. Vázquez-González, L. Sarmiento-Franco*, R. Santos-Ricalde, M. Carvajal-Hernández, J. Segura-Correa, M. Gutiérrez-Triay

Faculty of Veterinary Medicine and Animal Science

Universidad Autonoma de Yucatán

Apdo. Postal 4-116, Itzimna, Merida, Yucatán

Mexico

(Received May 16, 2007; accepted April 18, 2008)

Abstract

Vázquez-González, B., Sarmiento-Franco, L., Santos-Ricalde, R., Carvajal-Hernández, M., Segura-Correa, J. and Gutiérrez-Triay, M. 2008. Egg production and quality in laying hens fed on different digestible amino acid regimes and housed at three stocking densities. J. Appl. Anim. Res., 33: 175-180.

The objective of the present study was to compare the production performance and egg quality in hens fed on 3 different digestible amino acid regimes and housed at three stocking densities. One hundred and sixty two light Isa-Babcock B300 laying hens were allocated randomly according to 2, 3 or 4 birds per cage. The birds were fed on different diets: 70 (CP: 14.4%), 90 (CP: 17.37%) and 110% (CP: 21.58%) of essential digestible amino acids requirements. The effect of digestible amino acids concentration was significant (P<0.05) for egg weight, albumin weight and albumin width as well as N dropped and crude protein content of excreta. Hen stocking density affected (P<0.05) only feed intake. It is concluded that 90% of essential digestible amino acids requirements and 4 hens per cage density (40x40 cm) could contribute to reduce N pollution without adversely affecting egg production.

Key words: Egg Production, egg quality, digestible amino acids.

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Foetal Monitoring, Uterine Dynamics and Reproductive Performance in Spontaneous Farrowings in Sows

A. Olmos-Hernández1, M.E. Trujillo-Ortega2, M. Alonso-Spilsbury3, P. Sánchez-Aparicio3,

R. Ramírez-Necoechea3, D. Mota-Rojas3*

 

1Posgrado en Ciencias de la Producción y de la Salud Animal

FMVZ Universidad Nacional Autónoma de México (UNAM)

Ciudad Universitaria D.F.

México

2Animal Production Department: Swine

Facultad de Medicina Veterinaria y Zootecnia

Universidad Nacional Autónoma de México

Ciudad Universitaria D.F.

Mexico

3Animal Production & Agriculture Department

Research Area: Ecodesarrollo de la Producción Animal

Universidad Autónoma Metropolitana-Xochimilco

Mexico

(Received March 13, 2007, accepted April 14, 2008)

Abstract

Olmos-Hernández, A., Trujillo-Ortega, M.E., Alonso-Spilsbury, M., Sánchez-Aparicio, P., Ramírez-Necoechea, R. and Mota-Rojas, D. 2008. Foetal monitoring, uterine dynamics and reproductive performance in spontaneous farrowings in sows. J. Appl. Anim. Res., 33: 181-185.

To determine the farrowing process and to evaluate the effect of parity on uterine dynamics and reproductive performance, 120 Yorkshire X Landrace hybrid sows, twenty for each parity number from 1 to 6 were used. Frequency, duration and intensity of contractions were monitored as indicators of uterine activity, foetal cardiac frequency (FCF) was monitored to determine fetal distress. Overall, farrowing duration was 189 min, contraction number and intensity were 40.9 and 9.89 mm Hg, respectively. Sixth parity sows had the shortest labor duration. The average number of fetuses with severe bradycardia and distress was 0.5. There was a significant increase (P=0.001) in the number of uterine contractions in gilts and second parity sows. First parity sows showed the highest intensity of contractions (12.30 mm Hg, P=0.001) and the largest number of delayed FCF decelerations, (0.90, P=0.004), which agrees too with a large average number of intra-partum stillbirths (0.90) found. The present study establishes the basic uterine dynamics of eutocic parturitions which may help in swine obstetrics to design uterotonic treatments reducing the stillbirth rate due to asphyxia. These findings emphasize a need for farrowing supervision, especially for first and over fifth parity sows.

Keywords: Parity number, uterine dynamics, spontaneous farrowings, sow.

Effects of Feeding Phytogenic Substances and Phytase on Growth Performance and Nutrient Digestibility of Young Broilers

T.C. Loh1,*, H.G. Ling1, N.T. Thanh1, H.L. Foo2, M.A. Rajion3, S.I. David4

 

1Department of Animal Science

Faculty of Agriculture

2Department of Bioprocess Technology

Faculty of Biotechnology and Biomolecular Sciences

3Department of Preclinical

Faculty of Veterinary Medicine

Universiti Putra Malaysia

43400 UPM Serdang, Selangor

4Gladron Chemicals Sdn. Bhd., No. 7, Jalan TP7

UEP Industrial Park, 40400 Shah Alam

Malaysia

(Received September 14, 2007; accepted April 24, 2008)

Abstract

Loh, T.C., Ling, H.G., Thanh, N.T., Foo, H.L., Rajion, M.A. and David, S.I. 2008. Effects of feeding phytogenic substances and phytase on growth perfomrnace and nutrient digestibility of young broilers. J. Appl. Anim. Res., 33: 187-192.

The effect of feeding phytogenic feed additive (Biostrong®, BS510) and microbial phytase (Natuphos® 5000) on the growth performance and nutrient digestibility in broiler chickens was studied. A total of 280 male Ross broilers were raised from day old until 21 d of age under 3-tier cage system. The chicks were divided into 5 groups: Control – Basal diet; T1: Basal diet + phytase (using 100% matrix value of phytase); T2: Basal diet + BS510 (using 100% matrix value of BS510); T3: Basal diet + phytase (using 100% matrix value of phytase) + BS510 (without matrix) and T4: Basal diet + phytase (using 100% matrix value of phytase) + BS510 (using only 100% Ca, P matrix value of BS510). The birds fed with phytase or phytogenic substances or combinations had better growth performance than the control. The diets added with phytogenic substances and phytase had better digestibility of nutrients such as crude protein, phosphorus and calcium. It is concluded that both additives can be used in broiler chicken diet with advantage.

Key words : Phytogenic feed additive, phytase, nutrients, digestibility, broiler.

Mechanistic Model Evaluation of Tree Species and Cactae Commonly Consumed by Grazing Goats1,2

L. Vargas-Villamila,3, A. S. Juárez-Reyesb, J. Ku-Verac, E. Ávila-Valesd,4, F. Vargas-Villamile, S. Medina-Peraltad, M. A. Cerrillo-Sotob

aÁrea de Producción Animal

Colegio de Postgraduados Campus Tabasco

Periférico Carlos A. Molina Km. 3.5

Apartado Postal 24, 86500, H. Cárdenas, Tab. México

bFacultad de Medicina Veterinaria y Zootecnia

Universidad Juárez del Estado de Durango

Durango, Dgo. 34280, México

cFacultad de Medicina Veterinaria y Zootecnia

dFacultad de Matemáticas

Universidad Autónoma de Yucatán

Mérida, Yuc. 97110, México,

ePrograma de Matemáticas Aplicadas y Computación

Instituto Mexicano del Petróleo

07730, México, D.F.

(Received January 27, 2007; accepted April 10, 2008)

Abstract

Vargas-Villamil, L., Juárez-Reyes, A.S., Ku-Vera, J., Ávila-Vales, E., Vargas-Villamil, F., Medina-Peralta, S. and Cerrillo-Soto, M.A. 2008. Mechanistic model evaluation of tree species and cactae commonly consumed by grazing goats. J. Appl. Anim. Res., 33: 193-198.

The mechanistic dynamic model, Kóokay, was used to estimate the initial high degradable phase (QSC(0)), the medium degradation fractional rate (kND,NDSm), the fermentation fractional rate (kSm,SmF) and the relation between the produced gas as a function of the fermented mass (fΔG,ΔF). To obtain these parameters a bounded regression problem was solved using in situ and in vitro data. It was found that: the cactae had much higher value for QSC(0), kND,NDSm and kSm,SmF than tree leaves. The fΔG,ΔF > 40 mLG(mgF)-1 for Q. grisea and Opuntia spp and fΔG,ΔF < 40 mLG(mgF)-1 for the others. At the incubation time of 8 h, the fermentation of the tree leaves, the O. leptocaulis and the Opuntia spp was 20, ~35 and 60 mgF(100 mgDM)-1, respectively. It was concluded that kSm,SmF is an important parameter to determine the nutritive value of new feeds since it is useful to predict the F into the rumen. Also, the F for Q. grisea, Q. eduardii and A. schaffneri are similar, but lower than Opuntia spp and O. leptocaulis.

Key words: Modeling, tree, fermentation, degradation, cactae.

Gestation Duration and Rectal Temperature Changes During Peripartum Period in Dogs

A.K. Zonturlua*, O.A. Aksoyb, C. Kacarc

aDepartment of Obstetrics & Gynecology and Reproduction

Faculty of Veterinary Medicine, Harran University

Ôanl2urfa, Turkey

bGemlik Military Veterinary School and Training Center

Bursa, Turkey

cDepartment of Obstetrics and Gyneocology

Faculty of Veterinary Medicine, Kafkas University

Kars, Turkey

(Received November 13, 2007; accepted April 10, 2008)

Abstract

Zonturlu, A.K., Aksoy, O.A. and Kacar, C. 2008. Gestation duration and rectal temperature changes during peripartum period in dogs. J. Appl. Anim. Res., 33: 199-200.

To investigate duration of gestation and variation in body temperature during peripartum period 20 bitches (14 German Shepherds, GS and 6 Labrador Retrievers, LR) were used. Gestation duration was found to be 60.6±1.3 and 61.6±2.0 days in GS and LR, respectively. The rectal temperature, which was around 38.5C during 144 h before parturition decreased (P<0.05) to 37.4C at the onset of whelping. Thus, monitoring rectal temperature before expected date of parturition may help to predict the time of parturition.

Key words: Dog, gestation length, body temperature.

Laparoscopy for Direct Examination of Internal Organs in Small Animals

S.K. Maiti, A. Dutta, P. Ajith, N. Kumar, A.K. Sharma, G.R. Singh

Division of Surgery

Indian Veterinary Research Institute

Izatnagar-243122, India

(Received November 30, 2007; accepted March 16, 2008)

Abstract

Maiti, S.K., Dutta, A., Ajith, P., Kumar, N., Sharma, A.K. and Singh, G.R. 2008. Laparoscopy for direct examination of internal organs in small animals. J. Appl. Anim. Res., 33: 201-204.

The technique of laparoscopy was evaluated as a means of observing and examining the internal organs of small animals. Surgical planes of anaesthesia were obtained with standard combinations of xylazineketamine drugs in goats, dogs and rabbits. Three sizes of laparoscopes (2.7, 5.0 and 10.0 mm in diameter) were used in animals ranging from 0.8 to 30 kg body weight. The 5-mm laparoscope was the most versatile, although all laparoscopes allowed observation of different abdominal organs. A total of 230 examinations were performed in 15 goats, 68 dogs and 25 rabbits. Ten goats and 25 dogs underwent more than 3 or 5 examinations, respectively. Laparoscopy was simple, accurate and practical technique for visual examination of abdominal organs.

Keywords: Internal organs, laparoscopy, small animals, surgery.

Molecular Variability of Somatotropin Releasing Hormone (SRH) Gene in Mithun (Bos frontalis)+

Subodh Kumar*, Ram Prasath Manohar V.1, S.M. Deb2, Abhijit Mitra, Bejoy John,  Arjava Sharma, K.M. Bujarbaruah3

1Animal Genetics Division

Indian Veterinary Research Institute

Izatnagar-243 122, India

(Received November 08, 2006; accepted April 16, 2008)

Abstract

Kumar, S., Manohar, R.P.V., Deb, S.M., Mitra, A., John, B., Sharma, A. and Bujarbaruah, K.M. 2008. Genetic variability of somatotropin releasing hormone (SRH) gene in Mithun (Bos frontalis). J. Appl. Anim. Res., 33: 205-207.

A fragment of 452 bp corresponding to exon 2 and 3 of somatotropin releasing hormone (SRH) gene on amplification in 90 mithun (Bos frontalis) revealed two genotypic patterns viz. AA genotypic pattern (bands of 292 and 160 bp) and AB genotypic pattern (452, 292, and 160 bp) with respect to SauI restriction enzyme. The sequence of the A allele, which was the first report on mithun SRH gene, was submitted to GenBank (Accession No. EF014289).

Keywords: Bos frontalis, exon 2-3, mithun, PCR-RFLP, SauI, somatotropin releasing hormone.

 

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